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Innovative IVF Products

THE WELL OF THE WELL SYSTEM For Individual Embryo Culture

Individual culture of embryos of most mammalian species results in lower developmental rates  than culture of large groups. Zona-free embryos also have impaired developmental potential in  current culture systems. A solution for the problem is to produce small wells on the bottom of  a Petri dish or four-well dish by mechanical pressure with an appropriate iron rod or by  melting the bottom. Embryos are placed into the Well of the Wells (WOWs) individually (see the  picture below) and remain there for the whole culture period.  The system was found to be efficient to mimic the effect of communal cultures, increased the  developmental competence of zona-intact embryos, and supported the development of zona-free  embryos. Human experiments proved that higher pregnancy rates could be achieved with  blastocyst developed in WOWs.

The WOW culture system proves the importance of microenvironment in embryo development, and  offers a simple way to increase the overall efficiency of embryo production both in domestic  animals and humans.

Submarine Incubator

An eternal problem of all embryology laboratories is incubation. Commercially available  "refrigerator-type" large thermostats are unsuitable for the purpose, as frequent opening of  the doors required by the daily work disturb equilibrium of all cultures inside. Alternative  solutions are available, but their prices and running costs are rather high. However,  sometimes the less expensive solution is the best. Dishes containing embryos can individually  wrapped in laminated foil bags, filled with the desired gas mixture, heat-sealed and placed on  a shelves of simple non-gassed incubator or submerged into a water bath. The advantages of  this system are evident. Stability of temperature, humidity and gas mixture; quick after  opening - if opening required at all; flexibility in using different gas mixtures; safety;  cost efficiency; reduced contamination risks; few problems with cleaning; easy transport. 
Additionally, the culture system is also suitable for use under field conditions.

No computers, no LCD-s, no infrared sensors, no auto-sterilizing options, just reliability,  safety and extreme cost efficiency.

MACS GMP Annexin V Kit

  • Specific depletion of apoptotic spermatozoa
  • Sperm populations with with enhanced quality parameters
  • Improve sperm fertilization potential

Annexin V recognizes an antigen (externalized phosphatidylserine, EPS) in the plasma membrane of apoptotic as well as dead cells. For the depletion, cells are magnetically labeled with the MACS GMP Annexin V Reagent and passed through an MS Column. The magnetically labeled apoptotic or dead spermatozoa are retained in the column while the unlabeled living spermatozoa are collected in the flow-through for further processing. The MACS GMP Annexin V Kit components are free of human and animal-derived components. Product specifications are confirmed by batch-specific certificates of analysis and ensure consistency of quality. The MACS GMP Annexin V Kit shows no embryo toxicity, according to the Mouse Embryo Assay (MEA).

Robotic ICSI

Marksman's systems are capable of injecting suspended cells (e.g., zebrafish embryos and mouse oocytes/embryos) and adherent cells (e.g., endothelial cells and HeLa cells) with nano- to femto-liter biomolecules, at a high speed, and with minimal human intervention. The injection of a large number of cells (zebrafish embryos, mouse oocytes/embryos, and adherent cells) is required for applications such as embryonic development studies, mutation screening to identify gene functions, assisted reproduction, and molecule screening.

High-speed automated robotic microinjection Sun Y et al (2009) Annual meeting ASRM, P-214

Microfluidic Embryo Culture

Microfluidics is the technology of handling small volumes of liquid in microscopic environments. Microfluidic embryo perfusion culture is set to transform the way that embryo culture is performed, firstly in animal reproduction, and following that, in human IVF.



In vivo embryo culture:

This technology enables the embryos to start their life in close communication with the maternal environment. This is expected to increase the number of high-quality embryos and improve the success rate of the assisted reproductive cycle.


24Sure Genetic Screening for oocytes/embryos/Blastocysts

The new platform, called 24Sure, uses a bacterial artificial chromosome array and software to screen for abnormal or aneuploid chromosomes. First baby born using BlueGnomes 24sure single cell screening technology. Working in collaboration with the world leading IVF centre, CARE Fertility (Nottingham UK), BlueGnome 24sure microarray technology has been used for the first time to successfully screen polar bodies for all 24 chromosomes and select a euploid (normal chromosome content) egg that resulted in a successful pregnancy.

Multi Thermal Gradient (MTG) Freezing Technology

The MTG technology, also known as directional freezing, allows precise control over ice crystal propagation during the freezing process, thereby substantially reducing the mechanical damage caused to cells during freezing. 


Oocyte recovery, embryo development and ovarian function after cryopreservation and transplantation of whole sheep ovary.  Published January 2005  A. Arav, et.al. Human Reproduction Journal, 2005. Whole sheep ovary cryopreservation and transplantation. Published December 2004  A. Revel, M.D. et. al., FERTILITY AND STERILITY_VOL. 82, NO. 6, DECEMBER 2004.


In the ICSI procedure, an individual sperm is selected and injected into a oocyte. Until now, the only technique available to embryologists to select the sperm has been visual observation. With Biocoats new PICSI Sperm Selection Device embryologists are able to determine sperm selection in much the same way it happens in human biology. Sperm is placed in Biocoats laboratory PICSI dish containing samples of hyaluronan hydrogel. Hyaluronan is a naturally occurring biopolymer found in all human cells, including the gel layer surrounding the oocyte. 

Mature, biochemically competent sperm bind to the hyaluronan where they can be isolated by the embryologist and used for ICSI. The Biocoat device actually mimics a key step in the natural fertilization process, the binding of mature sperm to the oocyte complex. As a result, the selected sperm is essentially the same as one that would be successful in the natural reproductive process.



IMSI (intracytoplasmic morphologically selected sperm injection) is a modified form of the ICSI (intracytoplasmic sperm injection) technique, however the only difference is that it carefully selects the best quality sperm by magnifying 6000 times more than the conventional ICSI method. Patients with severe oligoasthenoteratozoospermia (low sperm count) can benefit from IMSI.


Embryo Box:

Magnetic flux: the quiet Killer  Is probably responsible for many failures in  the" In vitro fertilization" process.  Modern equipment contains heavy electronic circuits. Electronic heaters and fans increase dramatically the magnetic flux in incubators and on laminar work tables. Studies showed that embryos in their first 24 hours are most sensitive to magnetic radiation emitted from heated work surfaces and incubators. The embryo might be damaged at as low as 0.1 A/M magnetic field,  while the magnetic flux  inside some incubators reaches levels  as strong as 10 A/M. Until manufacturers will be more aware of that problem you can use a" Embryo Box" that stops the magnetic flux and gives embryos a better chance to develop normally.


Currently, assessment of embryo development in IVF is limited to brief snap-shot glimpses at defined time points, in order to minimize disturbances to culture conditions.  However embryo development is a dynamic process and a wealth of information about embryo history is sacrificed to avoid potentially damaging effect to embryo quality caused by removal from a controlled temperature and gas environment. The EmbryoScope Embryo Monitoring System provides a safe, controlled culture environment, while allowing continuous monitoring and observation of up to 72 embryos at a time. The instrument provides a unique opportunity to develop new parameters for future embryo scoring and improve embryo assessment in IVF.


Lopes, A.S., Greve, T. and Callesen, H. 2007. Quantification of embryo quality by respirometry. Theriogenology 67(1):21-31 (Entrez)


The technology, Amino Acid Profiling (AAP), is a quantitative, non-invasive method for selecting those embryos that are most likely to lead to a successful pregnancy and live birth.


ViaMetrics-E is a rapid, non-invasive procedure for in vitro fertilization (IVF) designed to aid in the assessment of viable embryos with the greatest reproductive potential. Through the use of a highly sensitive method of biomarker identification, ViaMetrics-E analysis can be performed on-site in just minutes, requiring only a small amount of spent culture media. ViaMetrics-E provides objective assessment of viability without compromising the embryo, helping guide treatment options for patients undergoing IVF.


PH Online:

pH is one of the most critical parameters regarding the performance of culture media. The embryo´s internal pH which is known to regulate various metabolic and transport processes can only be maintained in an appropriate environment. Typically, checking media pH by conventional pH meters is too cumbersome and time-consuming to be done in regular intervals.

pH OnlineTM is able to continuously monitor media pH in a reference dish placed in the incubator. Measurement is based on a non-contact, internally referenced dual luminophore technology which has huge advantages over conventional pH electrodes.

Rotationally Oscillating m -injector (Ros-Drill):

This procedure is based on a rotationally oscillating pipette motions during the drilling. These oscillations of small amplitudes (few degrees) and high enough frequencies (about 100 Hz) are shown to create very effective piercing. The so-called Ros-Drill© is a mercury-free and minimally invasive device of which the prototypes are designed and built including the relevant peripheral control hardware and software. Preliminary experiments have been done on mouse oocytes and Drosophila embryos. The resutls are very encouraging. In the early trials, blastocyst stage is reported using new drilling device on mouse and minimally invasive DNA micro-injection for Drosophila embryos.


Suvey on embryology profession/education New
Attention to all IVF lab professionals... We are c.....
Posted on : November 9, 2016   by:Dr. Alex Varghese

Dates 2015

4 - 6

Annual Meeting of the Middle East Fertility Society

Location: Liege, Belgium
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